Journal: Chemistry (Weinheim an Der Bergstrasse, Germany)

Article Title: Synthesis of 5‐Alkyl‐ and 5‐Phenylamino‐Substituted Azothiazole Dyes with Solvatochromic and DNA‐Binding Properties

doi: 10.1002/chem.201903657

Figure Lengend Snippet: Spectrophotometric titration of 7 b (1) and 8 b (2) with ct DNA [A, c L =10 μ m , c DNA =2.17 m m (A1), c DNA =1.49 m m (A2); c DNA in base pairs] in BPE buffer ( c Na+ =16 m m , pH 7.0; with 5 % v/v DMSO), 22AG (B, c L =5 μ m , c 22AG =285 μ m ; c 22AG in oligonucleotide) in K‐phosphate buffer pH 7.0 ( c K+ =73 m m ; with 5 % v/v DMSO) and PSS [C, c L =10 μ m , c PSS =190 μ m (C1), c PSS =195 μ m (C2)] in BPE buffer ( c Na+ =16 m m , pH 7.0; with 2.5–5 % v/v DMSO). Red: Spectra of the pure ligand solutions; blue: spectra at the end of the titrations. The arrows indicate the changes of absorption upon addition of the host molecule. Insets: Plot of Abs./ Abs. 0 versus c DNA / c L or c PSS / c L .

Article Snippet: The oligodeoxyribonucleotide 22AG d[A(GGGTTA) 3 GGG] (purification: HPLC; quality control: MALDI‐TOF; synthesis scale: 1.0 μmol) was purchased from biomers.net GmbH (Ulm, Germany).

Techniques: Titration

Journal: Chemistry (Weinheim an Der Bergstrasse, Germany)

Article Title: Synthesis of 5‐Alkyl‐ and 5‐Phenylamino‐Substituted Azothiazole Dyes with Solvatochromic and DNA‐Binding Properties

doi: 10.1002/chem.201903657

Figure Lengend Snippet: Absorption and Emission Properties of 7 b and 8 b in the Presence of ct DNA, 22AG and PSS.

Article Snippet: The oligodeoxyribonucleotide 22AG d[A(GGGTTA) 3 GGG] (purification: HPLC; quality control: MALDI‐TOF; synthesis scale: 1.0 μmol) was purchased from biomers.net GmbH (Ulm, Germany).

Techniques:

Journal: Chemistry (Weinheim an Der Bergstrasse, Germany)

Article Title: Synthesis of 5‐Alkyl‐ and 5‐Phenylamino‐Substituted Azothiazole Dyes with Solvatochromic and DNA‐Binding Properties

doi: 10.1002/chem.201903657

Figure Lengend Snippet: Spectrofluorimetric titration of 8 b with ct DNA (A, c L =10 μ m , c DNA =1.49 m m ; c DNA in base pairs) in BPE buffer ( c Na+ =16 m m , pH 7.0; with 5 % v/v DMSO), 22AG (B, c L =5 μ m , c 22AG =285 μ m ; c 22AG in oligonucleotide) in K‐phosphate buffer ( c K+ =73 m m , pH 7.0; with 5 % v/v DMSO) and PSS (C, c L =10 μ m , c PSS =195 μ m ) in BPE buffer ( c Na+ =16 m m , pH 7.0; with 2.5 % v/v DMSO); λ ex =515 nm. Red: Spectra of the pure ligand solutions; blue: spectra at the end of the titrations. The arrows indicate the changes in emission intensity upon addition of the host molecule. Insets: Plot of the relative fluorescence intensity I / I 0 (corrected with regard to the change of the absorption at the excitation wavelength) versus c DNA / c L or c PSS / c L . Inset pictures in A: Fluorescence colors of 8 b in the absence and in the presence of ct DNA; λ ex =366 nm. The contrast and brightness were enhanced by 30 % without changing the true colors (cf. Figure S5).

Article Snippet: The oligodeoxyribonucleotide 22AG d[A(GGGTTA) 3 GGG] (purification: HPLC; quality control: MALDI‐TOF; synthesis scale: 1.0 μmol) was purchased from biomers.net GmbH (Ulm, Germany).

Techniques: Titration, Fluorescence

Journal: PLoS ONE

Article Title: Quadruplex-forming oligonucleotide targeted to the VEGF promoter inhibits growth of non-small cell lung cancer cells

doi: 10.1371/journal.pone.0211046

Figure Lengend Snippet: Proliferation was measured by the MTT assay. (A) VEGFq, but not MutVEGFq inhibited the growth of A459 cells in a concentration and time dependent fashion. (B) VEGFq also inhibited the growth of the H1299 and H1944 NSCLC cell lines, H3255 squamous cell lung cancer cells, and the Calu-1 epidermoid carcinoma cell line, but had very little effect on the non-transformed foreskin fibroblast Hs27 cells and the lung epithelial cells, HPLD-1 measured at 144 h. Bars represent mean±SEM absorbance of three separate determinations. * indicates (p<0.05) compared to untreated cells.

Article Snippet: A549 (adenocarcinoma human alveolar basal epithelial cells), H1299 (lung adenocarcinoma), H1944 (lung adenocarcinoma), H3255 (lung adenocarcinoma), Calu-1 (epidermoid lung carcinoma) and as control nontransformed cells Hs27 (human fibroblast cells) and HPLD (immortalized human bronchiolar epithelial cells) (ATCC, USA) were maintained in DMEM media supplemented with 10% FBS and 100U penicillin/streptomycin at 5%CO2 and 37°C.

Techniques: MTT Assay, Concentration Assay, Transformation Assay

Journal: Communications Biology

Article Title: SARS-CoV-2 proteins and anti-COVID-19 drugs induce lytic reactivation of an oncogenic virus

doi: 10.1038/s42003-021-02220-z

Figure Lengend Snippet: a The iSLK.219 cells were transfected with vector control or vectors encoding SARS-CoV-2 spike protein (S), nucleocapsid protein (N) and KSHV RTA (as a positive control) with or without low dose of doxycycline (Dox, 0.1 µg/mL) induction for 72 h. The expression of RFP (representing viral lytic reactivation) and GFP (representing infected cells) were detected using fluorescence microscopy. Error bars represent S.D. for 4 image fields per well. b , c BCP-1 cells were transfected as above with or without low dose of 12- O -tetradecanoyl-phorbol-13-acetate (TPA, 1.0 ng/mL) induction for 72 h, then the transcripts of representative lytic genes were quantified by using qRT-PCR. The supernatants from transfected cells were collected to infect naive HEK293T cells, then viral genome levels were quantified by using qPCR with Lana -specific primers. Error bars represent S.D. for 3 independent experiments, ** p < 0.01 (vs the vector control). d Expression of ACE2 and LANA (as well as representative IgG control) in formalin-fixed paraffin-embedded KS tissues from 3 HIV+ patients and normal skin tissues were determined by immunohistochemical staining as described in the “Methods”. Bars: 50 μm.

Article Snippet: Human iSLK.219 cells were transfected with recombinant vectors of SARS-CoV-2 spike protein (S), nucleocapsid protein (N) (both purchased from Sino Biological), pCR3.1-RTA (a gift from Dr. Yan Yuan, University of Pennsylvania) , pFLAG-CMV2-p65 (pFLAG-p65, a gift from Dr. Ren Sun, University of California Los Angeles) , or vector controls, using Lipofectamine TM 3000 reagent (Invitrogen).

Techniques: Transfection, Plasmid Preparation, Positive Control, Expressing, Infection, Fluorescence, Microscopy, Quantitative RT-PCR, Formalin-fixed Paraffin-Embedded, Immunohistochemical staining, Staining